Long term immunologic consequences of experimental stroke and mucosal tolerance

Table 1 Immune status at 1 and 3 months following middle cerebral artery occlusion.

*time to sacrifice*	*1 month*			*3 months*			*1 vs. 3 months*
*brain*	*OVA* *N = 17*	*MBP* *N = 16*	P	*OVA* *N = 8*	*MBP* *N = 17*	P	*OVA*	*MBP*
T H 1	6 (35)	1 (6)	0.033	4 (50)	6 (35)	NS	NS	0.041
T REG	5 (29)	5 (31)	NS	0	3 (18)	NS	0.086	NS
*spleen*	*OVA* *N = 21*	*MBP* *N = 21*	P	*OVA* *N = 13*	*MBP* *N = 22*	P	*OVA*	*MBP*
T H 1	8 (38)	1 (6)	0.001	6 (46)	5 (23)	NS	NS	0.089
T REG	0	7 (33)	0.001	1 (8)	6 (27)	NS	NS	NS

Animals are considered to have a TH 1(+) response to MBP if the ratio of the relative increase in the number of MBP-specific IFN secreting cells to the relative increase in the number of MBP-specific TGF secreting cells is at least 1.48. Conversely, they are considered to have a TREG response to MBP if the ratio of the relative increase in the number of MBP-specific IFN secreting cells to the relative increase in the number of MBP-specific TGF secreting cells is ≤ 1 ÷ 1.48 (0.68).

ISSN: 2040-7378