Figure 1

Neurons express ADR1 and ADR2 and respond to oxygen and glucose deprivation. (A) ADR1 and ADR2 mRNA are present in cultured cortical neurons determined by single-cell RT-PCR analysis. The numbers indicate the number of neurons from which RNA was amplified; 1 and 3 cortical neurons consistently yielded a positive PCR signal for the ADR1 and ADR2 with exactly predicted size. (B) Cortical neurons subjected to OGD for the indicated times show increased levels of ADR1 and ADR2 mRNA in a time-dependent manner. (C) Immunoblot analysis of proteins in cell lysates of neurons in control cultures and cultures subjected to OGD for 3-24 h. OGD resulted in increased levels of ADR1 and ADR2 (D) ADR1 immunoreactivity (red) in cultured neurons; cells were counterstained with DAPI (blue) to label all nuclei. Arrow points to the axon of a neuron and arrowheads point to dendrites of the same neuron. (E) Cortical neurons subjected to OGD following globular adiponectin treatment show increased levels of ADR1.