Figure 3

ADR activation mediates neuronal cell death following in vitro ischemia-like conditions. (A, B) Neuronal cultures were treated with 10 μg of the globular adiponectin (gAd) and then subjected to OGD for 12 h (A) or GD for 24 h (B). Proteins in cell lysates were then subjected to immunoblot analysis by using the indicated antibodies. The gAd treatment enhanced OGD or GD-induced increases in levels of p-AMPK, p-38 MAPK and activated caspase-3. (C-D) Globular adiponectin (gAd) and trimeric adiponectin (tAd) treatment exacerbates OGD induced death of cultured primary neurons. Neuronal cell death was quantified 12 h later. Values are mean ± s.e.m. (n = 6-10 cultures). ***P < 0.0001 compared to OGD or vehicle treated OGD value, *P < 0.05 compared to OGD or vehicle treated OGD value. (E) Globular adiponectin (gAd) treatment exacerbates GD induced death of cultured primary neurons. Neuronal cell death was quantified 24 h later. Values are mean ± s.e.m. (n = 6-10 cultures). ***P < 0.0001 compared to GD or vehicle treated GD value.