Figure 2

Exemplary protocol of a series of ischemic tolerance (IT) experiments. Preconditioning ischemia (IPC) lasts 5 min, final ischemia 10 min. Cross indicates the end of the study where the brains are collected for ex vivo evaluations. Set I experiments evaluate early IT: final ischemia is applied 30 min after IPC (upper row), early ischemia-tolerant phenotype is tested by collecting the brains 25 min after PC, and in the control experiment, final ischemia is induced without prior IPC (lower row). In Set II experiments, delayed IT (upper row, final ischemia induction 24 h after the PC) and delayed ischemia-tolerant phenotype (middle row, brains are collected 24 h after PC) are investigated, along with a control experiment (lower row). Set III experiments address long-lasting effects of delayed-IT, with a follow-up lasting several weeks after final ischemia.