Brain structure volume estimates. Estimated volumes using the 2D nucleator and the Cavalieri principle are illustrated for hemispheres, subcortical structures, neocortices, and striatum. The presented volumes were estimated on NeuN-stained tissue sections on both sides in all sham animals and the mean volumes are summarized in Table 1. SD sham volumes are represented with "unfilled and filled triangles" for the left and right hemisphere, respectively. SHR sham volumes were likewise represented with "unfilled and filled diamonds". Mean volumes are marked with horizontal lines. NeuN, neuronal nuclei; SD, Sprague-Dawley rat; SHR, spontaneously hypertensive rat; tMCAo, transient middle cerebral artery occlusion.