Figure 2

HIF-1α is transiently induced whereas HIF-2α is continuously elevated in hypoxic hCMEC/D3 cells. Cells were exposed to normoxia (21% oxygen) or hypoxia (1%) for various hours. For chemical HIF-1α and HIF-2α induction, 0.1 mM of DFO or 1 mM of the prolyl-4 hydroxylase inhibitor dimethyloxaloyl glycine (DMOG) for 4 h were administered. HIF-1α and HIF-2α abundance was assessed by Western blot analysis.