Figure 3

ABCB1 and ABCC1 abundance is not influenced by hypoxia or anoxia in hCMEC/D3 cells. Cells were exposed to normoxia (21% oxygen), hypoxia (1%) or anoxia (0%, in A) for 4 to 48 hours. The abundance of ABCB1 and ABCC1 protein was evaluated by Western blot analysis. As positive control, cells exposed to the prolyl-4 hydroxylase inhibitor dimethyloxaloyl glycine (DMOG) were assessed (in B). Note the absence of expression changes at any of the time points examined (in A-C).