Figure 4From: The ATP-binding cassette transporters ABCB1 and ABCC1 are not regulated by hypoxia in immortalised human brain microvascular endothelial cells ABCB1 and ABCC1 expression is not influenced by hypoxia on the protein and mRNA level. Cells were exposed to normoxia (21% oxygen) or hypoxia (1%). The abundance of ABCB1 and ABCC1 protein was examined by Western blot analysis after 16 h (in A), whereas abcb1 and abcc1 mRNA levels were analysed by rt-PCR after 4 h exposure (in B). Data are means ± SD values. Results from four (in A) or three (in B) different experiments are shown. Rt-PCR data were normalised to sdha that was evaluated as housekeeping gene.Back to article page