Thromboembolic stroke model in C57BL/6 adult male mice. A) The clot is inserted at the MCA origin. Clots were visualized with Evan's Blue dye (arrowhead). B) Regional cerebral blood flow (CBF) over time as measured with Laser Doppler flowmetry (means ± SD, n = 7). The CBF declines to 18 ± 4% of baseline following embolization, which was associated with a reproducible sized infarct of the MCA territory (115 ± 22 mm3) at 24 post stroke hours. C, D) Representative infarct area at 24 hours as determined by T2 diffusion-weighted MRI (C) and with 2,3,5-triphenyltetrazolium chloride staining of the coronal brain sections (D). Additional representative images of thromboembolic stroke model are shown in the Additional file 1 (Figure S1), Additional file 2 (Figure S2), Additional file 3 (Figure S3), Additional file 9 (Additional Figure Legends) and Additional file 10 (Additional Methods).