Figure 6

Minocycline reduces level of MMP-9 in both genders. A) Densitometric analysis of immunoreactive band intensities and representative Western Blot showing expression of 92 kDa-MMP-9 in ipsilateral hemispheres of adult male and OVX female mice (n = 6-7 animals per group) at 6 hours after thromboembolization. Values are expressed as relative intensity normalized to 42 kDa-β-actin intensity. The saline-treated mice had higher levels of MMP-9 than minocycline treated animals (P < 0.0001, ***). Minocycline and sham groups were not significantly different from each other (P = 0.77). B) Densitometric analysis of brain MMP-9 activity and representative zymography. The brain injury shows increased variability of MMP-9 activity relative to sham, but there are no sign differences between treatment or sex groups. All data expressed as means ± SD.