| Dose-response study | Therapeutic window study | ||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
 | Saline | DHA 5 mg/kg | Alb 0.63 g/kg | DHA-Alb | Saline | DHA 5 mg/kg | Alb, 1.25 g/kg | DHA-Alb | Alb, 1.25g/kg | DHA-Alb | ||||||
 |  |  |  | 0.32 g/kg | 0.63 g/kg | 1.25 g/kg |  |  | 3h | 2h | 3h | 4h | 5h | 5h | 6h | 7h |
 | (n=6) | (n=7) | (n=6) | (n=7) | (n=5) | (n=7) | (n=7) | (n=8) | (n=8) | (n=8) | (n=8) | (n=7) | (n=9) | (n=7) | (n=9) | (n=8) |
Before MCAo (15 min) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Rectal temperature (oC) | 36.6 ± 0.1 | 37.3 ± 0.1 | 37.1 ± 0.1 | 36.9 ± 0.1 | 37.3 ± 0.2 | 37.1 ± 0.1 | 36.8 ± 0.1 | 36.9 ± 0.1 | 37.2 ± 0.1 | 37.0 ± 0.1 | 36.9 ± 0.1 | 36.9 ±0.1 | 37.0 ± 0.1 | 36.8 ± 0.1 | 37.0 ± 0.1 | 36.8 ± 0.1 |
Cranial temperature (oC) | 36.6 ± 0.1 | 36.8 ± 0.1 | 36.9 ± 0.2 | 36.3 ± 0.1 | 36.8 ± 0.2 | 36.6 ± 0.2 | 36.7 ± 0.3 | 36.8 ± 0.2 | 37.1 ± 0.1 | 36.9 ± 0.1 | 36.6 ± 0.2 | 36.8 ± 0.1 | 36.5 ± 0.2 | 36.5 ± 0.3 | 36.8 ± 0.1 | 36.7 ± 0.2 |
pH | 7.4 ± 0.03 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.5 ± 0.01 | 7.4 ± 0.01 | 7.5 ±0.01 | 7.5 ± 0.00 | 7.5 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.5 ± 0.01 | 7.4 ± 0.01 |
PO2, mm Hg | 120 ± 7 | 106 ± 7 | 115 ± 9 | 115 ± 7 | 113 ± 8 | 105 ± 5 | 102 ± 9 | 109 ± 6 | 104 ± 6 | 122 ± 10 | 96 ± 5 | 106 ± 7 | 102 ± 7 | 103 ± 6 | 108 ± 14 | 107 ± 10 |
PCO2, mm Hg | 40.0 ± 2.0 | 39.0 ± 1.0 | 38.8 ± 1.0 | 38.3 ± 0.7 | 39.4 ± 0.9 | 40.7 ± 0.5 | 37.5 ± 0.9 | 38.9 ± 0.7 | 37.6 ± 1.0 | 38.0 ± 0.9 | 39.0 ± 0.7 | 38.7 ± 0.8 | 38.6 ± 0.7 | 39.6 ± 1.6 | 47.3 ± 8.4 | 39.4 ± 1.0 |
Plasma glucose, mg/dL | 151 ± 14 | 154 ± 8 | 175 ± 13 | 181 ± 14 | 183 ± 18 | 193 ± 15 | 173 ± 13 | 132 ± 1.0 | 162 ± 14 | 161 ± 8 | 160 ± 7 | 147 ± 7 | 171 ± 10 | 156 ± 11 | 168 ± 11 | 156 ± 8 |
Hematocrit, % | 44 ± 1.0 | 43 ± 0.3 | 44 ± 0.9 | 44 ± 0.8 | 44 ± 0.8 | 44 ± 0.5 | 42 ± 1.0 | 45 ± 1.2 | 45 ± 1.2 | 43 ± 0.4 | 45 ± 1.2 | 43 ± 1.0 | 46 ± 0.7 | 44 ± 0.8 | 45 ± 12 | 43 ± 1.7 |
Body weight (g) | 334 ± 5 | 324 ± 8 | 304 ± 11 | 320 ± 7 | 309 ± 6 | 304 ± 7 | 334 ± 14 | 302 ± 4 | 327 ± 6 | 317 ± 9 | 306 ± 8 | 333 ± 7 | 335 ± 13 | 321 ± 11 | 326 ± 7 | 330 ± 11 |
During MCAo (15 min) | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Rectal temperature (oC) | 36.8 ± 0.1 | 37.2 ± 0.1 | 37.0 ± 0.1 | 37.0 ± 0.1 | 37.3 ± 0.1 | 37.1 ± 0.1 | 37.2 ±0.1 | 36.9±0.1 | 37.0±0.1 | 37.2±0.1 | 37.3±0.1 | 37.2±0.1 | 37.1±0.1 | 37.0±0.2 | 37.3±0.1 | 37.1±0.1 |
Cranial temperature (oC) | 36.6 ± 0.1 | 36.3 ± 0.1 | 36.6 ± 0.1 | 36.6 ± 0.1 | 36.7 ± 0.2 | 36.6 ± 0.1 | 37.0 03 | 37.0±0.0 | 37.6±0.5 | 37.0±0.0 | 37.0±0.2 | 36.9±0.1 | 36.9±0.1 | 36.9±0.1 | 36.6±0.2 | 37±0.0 |
pH | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.01 | 7.4 ± 0.02 | 7.5 ± 0.01 | 7.4 ± 0.1 | 7.4±0.01 | 7.4±0.01 | 7.5±0.02 | 7.4±0.02 | 7.4±0.02 | 7.4±0.00 | 7.4±0.01 | 7.4±0.01 | 7.4±0.02 |
PO2, mm Hg | 112±8 | 97 ± 5 | 99 ± 7 | 104 ± 5 | 108 ±7 | 99 ± 4 | 98 ± 1 | 98±4 | 103±5 | 105±8 | 96±5 | 107±9 | 113±9 | 101±6 | 102±6 | 97±6 |
PCO2, mm Hg | 40.0 ± 2.0 | 40.0 ± 0.0 | 39.2 ± 0.8 | 40.7 ± 1.0 | 39.0 ± 0.9 | 38.4 ± 0.8 | 41.0 ± 1.4 | 39.2±0.6 | 39.1±0.7 | 39.8±1.0 | 40.0±1.3 | 39.5±0.8 | 39.7±0.6 | 39.8±0.6 | 39.3±0.7 | 40.3±1.7 |
Plasma glucose, mg/dL | 141 ± 7 | 159 ± 7 | 176 ± 13 | 181 ± 14 | 202 ± 19 | 189 ± 9 | 160 ± 10 | 148±15 | 150±11 | 156±9 | 145±27 | 151±9 | 169±11 | 159±10 | 149±8 | 147±8 |
Hematocrit, % | 44 ± 1.0 | 43 ± 0.4 | 44 ± 1.0 | 44 ± 0.7 | 43 ± 0.4 | 44 ± 0.5 | 43 ± 1.4 | 46±0.5 | 44.6±1.4 | 45±0.8 | 46±0.9 | 44±1.0 | 46±0.9 | 44±1.1 | 47±1.1 | 46±1.3 |
15 min after treatment | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Rectal temperature(oC) | 37.2±0.3 | 36.6±0.2 | 37.2±0.1 | 37.2±0.3 | 37.1±0.1 | 37.0±0.1 | 36.7±0.1 | 37.0±0.1 | 37.2±0.2 | 36.7±0.1 | 37.0±0.2 | 37.1±0.1 | 37.1±0.2 | 37.1±0.2 | 37.1±0.2 | 37.2±0.1 |
Cranial temperature(oC) | 37.2±0.2 | 36.6±0.2 | 37.5±0.2 | 37.9±0.4 | 37.7±0.3 | 37.2±0.2 | 36.1±0.1 | 36.1±0.1 | 36.8±0.3 | 36.3±0.2 | 36.4±0.3 | 36.3±0.2 | 36.8±0.2 | 36.3±0.3 | 36.1±0.1 | 36.8±0.2 |
Hematocrit, % | 45±1.1 | 44±0.9 | 37±0.3 | 40±0.6 | 37±0.5 | 35±0.7* | 43±1.4 | 44±1.0 | 35±1.4* | 35±0.8* | 38±0.6* | 35±0.6* | 36±0.8* | 36±0.8* | 36±0.6* | 36±1.1* |
1 day after treatment | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Rectal temperature (oC) | 38.3±0.2 | 38.3±0.2 | 38.4±0.1 | 37.8±0.2 | 38.0±0.3 | 37.7±0.1 | 38.0±0.2 | 38.0±0.2 | 38.1±0.3 | 37.8±0.5 | 37.0±0.2 | 37.7±0.3 | 38.0±0.3 | 37.6±0.2 | 37.8±0.1 | 37.7±0.2 |
Body weight(g) | 301±6 | 300±7 | 270±10 | 294±7 | 287±7 | 281±6 | 299±14 | 275±7 | 304±9 | 303±10 | 283±10 | 304±10 | 303±15 | 294±16 | 308±9 | 303±14 |
7 days after treatment | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â |
Rectal temperature (oC) | 37.8±0.3 | 38.0±0.2 | 35.1±1.4 | 37.6±0.3 | 37.8±0.2 | 37.8±0.1 | 37.7±0.1 | 37.5±0.2 | 37.7±0.2 | 37.7±0.1 | 36.7±0.9 | 37.4±0.5 | 35.8±1.3 | 37.2±0.2 | 37.4±0.1 | 36.4±1.2 |
Hematocrit, % | 44±1.3 | 40±0.8 | 47±0.2 | 41±0.7 | 45±0.5 | 43±0.8 | 45±1.2 | 41±1.1 | 40±1.1 | 42±0.9 | 39±0.5 | 39±0.3 | 41±0.8 | 39±0.8 | 40±0.7 | 39±1.0 |
Body weight (g) | 305±16 | 319±17 | 219±12 | 305±19 | 307±12 | 310±7 | 318±18 | 297±16 | 331±14 | 294±25 | 311±20 | 307±21 | 333±24 | 304±24 | 345±7 | 321±21 |