Ischemic injury from ET-1 injection is greater in hENT1 Tg mice compared to Wt littermates. Ischemic infarct size was determined from T2 weighted MRI at 48 hours post injection of either saline or ET-1. Two-way ANOVA indicated a significant drug effect (F(1, 27) = 22.3, p < 0.001). Bonferroni post-tests indicated no difference between Wt and hENT1 Tg mice injected with saline but a significantly larger infarct in hENT1 Tg, relative to Wt mice, following ET-1 injection (p < 0.05). Data are means ± SEM, n = 12 Tg ET-1, 13 Wt ET-1, 4 Tg saline and 4 Wt saline.