Figure 3From: Coadministration of FTY720 and rt-PA in an experimental model of large hemispheric stroke–no influence on functional outcome and blood–brain barrier disruption FTY720 does not enhance blood–brain barrier patency in large hemispheric infarcts neither alone nor in combination with rt-PA. A) For the determination of wet brain weight, the cerebellum was discarded and brain hemispheres were frozen separately at −80°C. The frozen brain hemispheres were weighed prior to homogenization. Only mice whose ischemic hemisphere (I) showed an increase of wet brain weight of ≥ 10% in comparison to the non-ischemic hemisphere (NI) were included in our analysis. B) EB (200 μl of a 2% solution) was injected 1 h prior to sacrifice and transcardial perfusion. We excluded mice who showed more EB extravasation into the NI hemisphere and mice who died within the EB circulation time of 1 h. Statistical significance of the differences between groups was tested with One-way ANOVA with Bonferroni correction. ns indicates not significant.Back to article page