Infarct volumes 24 h after 30 min MCA occlusion in hcn2+/+
mice. (A) Representative 3,5-Triphenyltetrazoliumchloride (TTC) stained images of three corresponding coronal sections (upper panel) of control (hcn2+/+) and hcn2-/- mice. Ischemic infarctions appear white and regularly and include the neocortex and basal ganglia as confirmed by haematoxylin and eosin staining (lower panel) (bar: 1 mm). (B) Mean brain infarct volumes on day1 after 30 min tMCAO in the two animal groups (hcn2+/+: n = 11; hcn2-/- mice: n = 11). Non-significant differences (ns), unpaired, 2-tailed Student’s t-test.