Assessment of the cerebral vasculature in hcn2-/-
littermates. (A) Regional cerebral blood flow (rCBF) was monitored using the Laser-Doppler flowmetry prior (baseline), during (ischemia) and shortly after (reperfusion) 60 min of tMCAO to make sure that perfusion deficits were similar in hcn2-/- (grey) and hcn2+/+ mice (black; n = 3 per group). No differences in rCBF were observed between the groups at any time point, ns: not significant 2-way ANOVA, Bonferroni‘s post hoc test. (B) Assessment of the cerebral vasculature in hcn2-/- and control (hcn2+/+) littermates by perfusion with black ink. A complete Circle of Willis was found in both animal groups and the anatomy of the MCA trunk and branch was identical in hcn2+/+ and hcn2-/- mice.