Visualisation of vascular filling and clot presence (Study 2). Vessels were perfused post-mortem with Microfil (yellow) to visualise the vasculature and clot presence (black). All animals had clot in the major cerebral vessels after treatment. (A) Shows the vasculature from the view of the Circle of Willis, (B) shows the lateral surface of the right hemisphere. Vessels labelled are: middle cerebral artery (MCA), anterior cerebral artery (ACA) and internal carotid artery (ICA). Images of all brains can be viewed in the Additional file 1.