Figure 2

Cerebral ischemia increases ADRs immunoreactivity in the brain. (A) Immunoblot analysis of protein samples from the cerebral cortex of sham operated control mice and mice subjected to 1 h cerebral ischemia and 1-24 h reperfusion. Ischemia resulted in rapid increases in the levels of ADRs immunoreactivities in neurons in the penumbra area (P). (Scale bars: 50 μM). (B) Images of brain sections showing ADR1 immunoreactivities (green) and NeuN (neuronal marker) in mice subjected to cerebral ischemia (1 h) and reperfusion (24 h). (C) Adiponectin accumulates in vessels like structures (large yellow arrow) and in parenchyma (small yellow arrow) in the human ischemic brain. (D) Control brain tissue stained with secondary antibody shows no staining.