Neuroglobin (Ngb) expression and infarct distribution. Ngb expression (brown) and infarct distribution on Haematoxylin counter stained sections 24 hours after permanent middle cerebral artery occlusion (pMCAo). A. Uninjured, B. wild-type (WT) pMCAo and C. Ngb-null pMCAo. Note that there is no Ngb staining in Ngb-null mice, and that staining in cortex is very limited. The infarct is marked with red lines on the sections within red squares, which are shown in higher magnification in Figure 2.