Immunohistochemical staining for Ngb in the brain. Immunohistochemical staining for Ngb in the brain of Ngb +/+ and Ngb -/- mice. In house made rabbit antibody 4836/5 showed specific (Ngb) staining (black arrow, signifying specific staining) in Ngb +/+ mice (A, C), which was abolished in Ngb -/- mice (B, D). Sigma rabbit Ngb antibody N7162 stained most neurons of cortex, hippocampus (white arrows, signifying unspecific staining) and neurons of the laterodorsal tegmental nucleus (LDTg) (black arrow) (E, G). Only the staining in the large LDTg neurons was abolished in Ngb -/- mice (F, H). No specific staining was observed using the Santa Cruz sc-22001 antibody (I-L). Dentate gyrus (DG). Scale bar 50 μm.