STL- (white), Glutathione- (red) and GFAP- (green) immunohistochemistry in a treated SHRSP. Glutathione is produced by astrocytes juxtapositioned to the small vessels’ endothelial cells (A &C). Comparing both groups, the treated one exhibited significant more Glutathione-positive vessels surrounded by astrocytes (D, histogram, y-axis with mean ratio of Glutathione-positive vessels in %). B – Negative control for Glutathione (Cy3 donkey x mouse) and GFAP (Cy5 donkey x chicken). DAPI - 4',6-diamidino-2-phenylindole, fluorescent stain for DNA detection. A, C hippocampal arteriole of a 26 weeks old SHRSP, magnification 630, ** p ≤ 0.05.