Serial sections of reactive zone surrounding infarct show glial scar formation. Hematoxylin and eosin stained sections show hypercellularity and loss of normal neuroparenchymal architecture in the reactive zone surrounding the infarct center (A). GFAP staining in serial section showed increased cellularity due to increased numbers (astrocytosis) and size (astrogliosis) of astrocytes indicating glial scar formation (B). Serial sections stained for Olig2 showing presence of oligodendrocytes (brown nuclear staining) with enlarged nuclei (C). MBP staining (red) showing loss of myelin and remnant myelin is seen as globular staining rather than linear (D). B and C: DAB chromogen with hematoxylin counterstain. E: Fast red chromogen with hematoxylin counterstain. Bars = 100 m.