Enhancement of autophagy with rapamycin decreases infarct size in MCAL mouse models of ischemic stroke injury. A). 8–12 week old male C57BL/6 J mice underwent MCAL stroke injury, and were treated twice (0 and 24 hours) with either saline, rapamycin (rapa) to enhance autophagy, chloroquine (CQ) to inhibit autophagy, or a combination of rapa and CQ. Infarct size analysis using 2% TTC staining (n = 9 mice per group). B). Quantitation of panel A data (* = p < 0.05). Note tissue drop out in the lesion site is due to the fragility of the tissue, not surgical damage.